Zygotes are single cell that is generated after the egg has been fertilized by the sperm. The zygote then merges chromosomes from both of the gametes. After which, several divisions will take place for the production of embryonic cells at roughly four days after fertilization. The zygote stage lasts for only about 4 days in total. On and around the 5th day, the cell mass of become known as blastocysts.
Culture medium also called growth medium pertains to the liquid medium necessary to carry out a culture and sensitivity test. The substance acts as a mode of support and nourishment for the cells undergoing culture, and may also contain growth factors necessary to generate cell change.
Request InformationAdipose tissue pertains to fat, which is primarily made up of fat cells known as adipocytes. [1]
Adipose stem cells or “ASCs” are an source of therapeutic mesenchymal cells for younger patients or those seeking cosmetic treatments like stem cell breast treatment and stem cell facelifts. Unlike the limited quantities of bone marrow derived stem stem cells “BMSCs” The adipose tissue or “Fat Stem Cells” can be extracted in fairly large quantities using special mini-liposuction aspiration before they are expanded in a special stem cell lab for several days in vitro.[2] Adipose fat stem cells and Preadipocytes are useful in treatments since they have the ability to home and undergo osteogenic, adipogenic, chondrogenic, and even neurogenic differentiation using our proprietary growth medium. Adipose stem cells are also immunoprivileged and have virtually zero chance of rejection thus preventing any risk of getting graft-versus-host disease. [3]
Despite the promising results, however, fat stem cells and lipofilling can provide unpredictable graft retention rates unless enriched/cultured for several days. Without proper cell culturing the reabsorption rates can be very high varying from 40% to 85% [4]
Adipose Fat Cells Under Microscope
Adipose tissue can be found in pockets of fat near the lower abdomen and inner thigh regions only. The tissue is believed to have adult stem cells; thus, its common use in cosmetic stem cell therapies. The extraction and isolation of adipose “fat” stem cells are usually performed through a closed-system mini-liposuction and the expansion with cell culturing medium requires several days before they can be reintroduced to the patient for variety of treatments including peripheral neuropathy, heart attacks, strokes and Coronary artery disease (CAD).
To learn more about our Enriched Adipose tissue derived stem therapies please contact us today.
[1] ^ Harasymiak-Krzyżanowska, Izabela, Alicja Niedojadło, Jolanta Karwat, Lidia Kotuła, Paulina Gil-Kulik, Magdalena Sawiuk, and Janusz Kocki. 2013. Adipose tissue-derived stem cells show considerable promise for regenerative medicine applications. Cellular & molecular biology letters, no. 4 (August 14). doi:10.2478/s11658-013-0101-4. https://www.ncbi.nlm.nih.gov/pubmed/23949841
[2] ^ Mohammadi, Zahra, Jalil Tavakkol Afshari, Mohammad Reza Keramati, Daryoush Hamidi Alamdari, Meysam Ganjibakhsh, Azam Moradi Zarmehri, Ali Jangjoo, Mohammad Hadi Sadeghian, Masoumeh Arab Ameri, and Leila Moinzadeh. 2015. Differentiation of adipocytes and osteocytes from human adipose and placental mesenchymal stem cells. Iranian journal of basic medical sciences, no. 3. https://www.ncbi.nlm.nih.gov/pubmed/25945239
[3] ^ Salyutin, R V, K M Zapohlska, S S Palyanytsya, V M Sirman, and M F Sokolov. 2015. [Differentiation of mesenchymal stem cells of adipose tissue]. Klinichna khirurhiia, no. 3. https://www.ncbi.nlm.nih.gov/pubmed/26072548
[4] ^ Zanetti, Andrea S, Gregory T McCandless, Julia Y Chan, Jeffrey M Gimble, and Daniel J Hayes. 2013. In vitro human adipose-derived stromal/stem cells osteogenesis in akermanite:poly-ε-caprolactone scaffolds. Journal of biomaterials applications, no. 7 (June 24). doi:10.1177/0885328213490974. https://www.ncbi.nlm.nih.gov/pubmed/23796629
Autologous stem cells such as Adipose tissue derived stem cells are the opposite of allogenic stem cells, as only one patient stands as both donor and receiver.
Autologous Stem Cell Therapy
The stem cells will be harvested from the patient’s body, most common location of which is the bone marrow, and will be transplanted back to the patient for treatment of a variety of conditions including: shoulder injuries, Acute coronary syndrome,diastolic heart failure, als fasciculations and anoxic brain injuries.
Ancestor cell or precursor cell are generic term for a cell that has no capability to renew itself, although it has been found to generate tissue stem cells in some cases, thus contributing to the formation of tissues.
Amniotic membrane and fluid derived Stem cells (AFS) are considered a powerful and non-controversial resource for cells that are used in transplantation therapy, mainly due to their extremely high renewal capacity
Amniotic derived cells are found in the amniotic fluid and membrane. They are also considered adult stem cells and are known to have a multifunctional capacity and multiple potency as well, as these can develop into osteogenic, myogenic, adipogenic, hepatic, neuronal, and endothelial cells.[1]
Amniotic derived stem cells are the result of a unique mixture of cells from both amniotic fluid as well as the amniotic membrane. These powerful cells are acquired without the objections of embryonic derived stem cells yet are extremely powerful for use in Regenerative therapies. These cells can be differentiated into many tissue types for treatment in skin disorders, cartilage damage, cardiac tissue and heart disease, Neuro conditions like MS,ALS, MND, muscle damage and even bone.[2]
The most important characteristics and benefits of enhanced AFS’s is that they are non-cancerous ( teratomas ) when transplanted to patients and are characterized by a very low antigenicity which represents a big advantage for our cell transplantation therapies. The Amniotic cells are isolated during the process of amniocentesis which means they are free from the ethical constraints and do not injure the fetus in any way.[3]
[1] ^ A M Ghanem, F M Zaher, A G Salman, R M Nabil, Comparison between limbal stem cell versus dry amniotic membrane transplantation in treatment of primary pterygium, QJM: An International Journal of Medicine, Volume 113, Issue Supplement_1, March 2020, hcaa058.032, https://doi.org/10.1093/qjmed/hcaa058.032
[2] ^ Borzou, B., Mehrabani, D., Zare, S., Zamani-Pereshkaft, M. and Acker, J.P., 2020. The Effect of Age and Type of Media on Growth Kinetics of Human Amniotic Fluid Stem cells. Biopreservation and Biobanking. https://doi.org/10.1089/bio.2019.0103
[3] ^ Harrell, C.R., Fellabaum, C., Markovic, B.S., Arsenijevic, A. and Volarevic, V., 2019. Therapeutic potential of “Exosomes derived Multiple Allogeneic Proteins Paracrine Signaling: Exosomes d-MAPPS” is based on the effects of exosomes, immunosuppressive and trophic factors. Serbian Journal of Experimental and Clinical Research, 20(3), pp.189-197. https://doi.org/10.2478/sjecr-2018-0032